Overview to scale the picture below is a simulation at scale of the waters xevo g2 xs qtof lcms mass spectrometer system right side connected with an acquity uhplc left side, installed on a ionbench ms mass spec bench or table. Xevo g2s tof is designed for scientists who need to identify and quantify the broadest range of compounds in complex and challenging samples. Uplcqtofms analysis of extracts from the leaves of. Sep 14, 2011 watch this xevo g2 qtof animation to learn how you can obtain the highest quality, most comprehensive information that will enable you to make the right decisions quickly and confidently. Comparison of fragmentation experiments for the structural. Xevo g2xs qtof laboratory equipment pdf manual download. Waters introduces xevo g2xs qtof and xevo tqs micro mass. In order to characterize the chemical profiles of different geographical origins of rpm samples, ultrahigh performance liquid chromatography quadrupole time of flight mass spectrometry uplcqtofms combined with chemometrics partial least squared. S5 the mass spectrometer was run in sensitivity positive ion mode with source parameters as follows.
The true benchtop stature of the x500b qtof system occupies less of your valuable lab space than any other high resolution mass spectrometry system on the market today. Tof rf settings positive ion mode collision cell system 1 6. Data file naming is always qtof101, qtof102 etc where the 11 implies the year 2011 in this case. Proposed billing codes and rates, effective january 2014. Intact trastuzumab monoclonal antibody quantification. The xevo g2 xs tof combines stepwave ion optics, xs collision cell, and quantof technology for superior robustness, sensitivity, and selectivity along with dynamic range, mass accuracy, and speed of analysis, resulting in the highest quality, most comprehensive qualitative and quantitative information. In the ewre lab we have a watersmicromass xevo g2xs qtof, quadrupole timeofflight mass spectrometer. From the front end all the way through the tof path, see how the x500b system is built for reliability. The liquid chromatography flow was split and infused at 500 nlmin into a xevo g2xs tof mass spectrometer waters by a triversa nanomate robot advion. The xevo g2xs tof combines stepwave ion optics, xs collision cell, and quantof technology for superior robustness, sensitivity, and selectivity along with dynamic range, mass accuracy, and speed of analysis, resulting in the highest quality, most comprehensive qualitative and quantitative information. Mass measurement the mass measurement accuracy of the instrume nt, in resolution mode, will be. Considerations specific to the xevo g2 s qtof solventleakage hazard the source exhaust system is designed to be robust and leaktight.
Waters recommends you perform a hazard analysis, assuming a maximum leak into the laboratory atmosphere of 10% lc eluate. An intelligentized strategy by lcesi q tof mse was presented in this study for rapid identification of hemostatic chemical constituents from. For the scientist who needs to identify, quantify and confirm the broadest range of compounds in the most complex and challenging samples the xevo g2xs tof provides maximum robustness with no compromise in performance, the highest quality, most comprehensive qualitative and quantitative information. Increasing numbers of compounds requiring stability data means. Waters acquity hclass uplc with pda and column manager. A 1259 2012 315 5 table 3 overview of commercial mass spectrometers designed for maldims with their technical speci. Exports results in an html, spreadsheet or as a tab delimited file and generates a formatted. The xevo g2xs qtof combines stepwave ion optics, xs.
Nitrogen used as cone and desolvation gas were set to 30 and 800lh, respectively. The extract in ethanol was obtained by maceration at room temperature and subjected to the liquidliquid partition to obtain fractions in hexane and ethyl acetate. The xevo g2 xs qtof combines stepwave ion optics, xs collision cell, and quantof technology for superior robustness, sensitivity, and selectivity along with dynamic range, mass accuracy, and speed of analysis, resulting in the highest quality, most comprehensive qualitative and quantitative information. Msms tof mrm scan time 250 ms mass range 70400 mz mass resolution 22000 acquisition time.
Msms tof mrm scan time 250 ms mass range 70400 mz mass resolution 22000 acquisition time 5 min lockmass acquisition. Intact trastuzumab monoclonal antibody quantification using. How to reboot the epc on a xevo g2xs wkb56994 waters. Quantitative performance attributes including sensitivity, accuracy. Jun 15, 2017 toddalia asiatica root bark as an effective hemostatic natural medicine or chinese materia medica was applied in china for long history, its complex drug action mechanisms and unclear substance basis have been constraining the development of this drug. For the scientist who needs to identify, quantify and confirm the broadest range of compounds in the most complex and challenging samples the xevo g2 xs tof provides maximum robustness with no compromise in performance, the highest quality, most comprehensive qualitative and quantitative information. The xevo g2 xs qtof delivers not only conventional ms and msms methods of data acquisition, but also mse for comprehensive accurate mass precursor and fragment ion information from a single analysis, fastdda for rapid, intelligent automated accurate mass msms, and tof mrm for enhanced quantitative capability. The folindenis assay is a fast and simple method for determining the phenolic compound contents in plant samples. Quantitation of high resolution ms data using unifi waters.
An intelligentized strategy by lcesi qtof mse was presented in this study for rapid identification of hemostatic chemical. Medical swab analysis using desorption electrospray ionization mass spectrometry desims a novel noninvasive approach for. Comparison of abundance of selected peaks significantly differing. The xevo g2xs qtof combines stepwave ion optics, xs collision cell, and quantof. Fully annotated 2d glycan structure view along with their glycosidic and cross ring fragments. The picture below is a simulation at scale of the waters xevo g2 tof lc msmass spectrometer system right side connected with an acquity uhplc left.
In detail, the instrument is connected to a waters. Hemostatic chemical constituents from natural medicine. The instrument is developed to give the most accurate and reproducible separation of complex sample matrices. Ambient mass spectrometry as a tool for a rapid and. Nowadays, radix polygoni multiflori rpm, heshouwu in chinese from different geographical origins were used in clinic. Xevo g2 xs tof performance, robustness, and flexibility made accessible to everyone. Mass spectrometry was performed on the xevo g2 q tof ms waters equipped with the electronic spray ion esi under negative ion mode. The xevo g2 xs qtof is for research use only and is not intended for use in diagnostic applications. Uplcqtofms analysis of extracts from the leaves of pouteria.
Maintenance services for xevo g2s tof mass spectrometer description and quantity of items. The extraction cone, sampling cone and capillary voltages were 4, 40 and 3000 v, respectively. Intended use of the xevo g2 xs qtof waters designed the orthogonal acceleration, timeofflight xevo g2 xs qtof for use as a research tool to deliver authenticated mass measurement. Waters xevo g2xs qtof overview and maintenance manual pdf. Medical swab analysis using desorption electrospray. We are experiencing some noise problems with our new qtof mass spectrometer waters xevo g2xs. This study describes the phytochemical profile and antioxidant activity of an extract from the leaves of pouteria caimito sapotaceae. Engineered for standard biotherapeutic characterization analyses, the x500b qtof system is a true benchtop instrument, with streamlined processing to get to a consistent, accurate view of your biologic. Recently, for quantitative analysis, waters introduced a new targetenhancement mode of data acquisition, tofmrm, on the synapt g2s hdms and xevo g2xs systems. For scientists who need to confidently identify and quantify unknown compounds in complex samples, the xevo g2 tof mass spectrometer with uplcmse and quantof technology offers exceptional levels of selectivity, inspectrum dynamic range, speed. Watch this xevo g2 qtof animation to learn how you can obtain the highest quality, most comprehensive information that will enable you to make the right decisions quickly and confidently. Lewis, paolo inglese, goncalo ds correia, trevor t. Medical swab analysis using desorption electrospray ionization mass spectrometry desims a novel noninvasive approach for mucosal diagnostics pamela pruski, david a.
A central cysteine residue is essential for the thermal. The liquid chromatography flow was split and infused at 500 nlmin into a xevo g2 xs tof mass spectrometer waters by a triversa nanomate robot advion. Flip down the electronics and epc breaker switch to turn off the ms. In this mode, a precursor ion is selected by the quadrupole and, optionally, fragmented in the collision cell. Waters advances proteomics and lipidomics research with. The waters xevo g2xs qtof is a high performance benchtop qtof mass spectrometer incorporating the xs collision cell for enhanced sensitivity and selectivity, resulting in the highest quality most comprehensive qualitative information together with the. Steroids, triterpenes, saponins, alkaloids and flavonoids were identified by the phytochemical. Mass spectrometry was performed on the xevo g2 qtof ms waters equipped with the electronic spray ion esi under negative ion mode. Xevo g2 xs tof is a benchtop time of flight ms combining stepwave ion optics, xs collision cell and quantof technology for superior robustness, sensitivity and selectivity, resulting in high quality, comprehensive qualitative and quantitative information. Endopeptidase regulation as a novel function of the zur. Ionbench with the waters xevo g2 tof mass spectrometer. We are experiencing some noise problems with our new qtof mass spectrometer waters xevo g2 xs. In order to characterize the chemical profiles of different geographical origins of rpm samples, ultrahigh performance liquid chromatography quadrupole time of flight mass spectrometry uplcqtofms combined with chemometrics partial least squared discriminant analysis.
Software control of gas flows and heating elements. Intended use of the xevo g2xs qtof waters designed the orthogonal acceleration, timeofflight xevo g2xs qtof for use as a research tool to deliver authenticated mass measurement. Using uplcqtofms method to analyse the spot constituents of. It shows how to import a workflow, and also briefly describes the investigate and report tabs. Stepwave ion optics provides unsurpassed levels of durable sensitivity. The xevo g2xs qtof mass spectrometer combines the new xs collision cell with the proven, signature technologies of tofmrm, stepwave tm. In the ewre lab we have a watersmicromass xevo g2 xs qtof, quadrupole timeofflight mass spectrometer. For a single sample run you can use the following procedure or you can skip and use the worklist. Maintenance services for xevo g2s tof mass spectrometer. Jun 16, 2014 the xevo g2 xs qtof mass spectrometer combines the new xs collision cell with the proven, signature technologies of tof mrm, stepwave tm and quantof tm to deliver optimum levels of robust. For further information, see the waters xevo g2xs qtoftof site preparation guide part number 715003608. Project management content management system cms task management project portfolio management time tracking pdf. Route the open end of the exhaust tubing to a suitable exhaust vent. On the ms, flip the pump override switch to the right, to protect the vacuum assuming the unit is still pumped down.
Fsu college of medicine translational science laboratory. The xevo g2xs qtof combines stepwave ion optics, xs collision cell, and quantof technology for superior robustness, sensitivity, and selectivity along with dynamic range, mass accuracy, and speed of analysis, resulting in the highest quality, most comprehensive qualitative and quantitative information. A tryptic digest of a monoclonal antibody was spiked with low levels of a stableisotobe labelled peptide. The waters acquity uplc iclass ensures fast analysis with high resolution power.
Compared to our older generation synapt g2 hdms, the xevo g2x2 qtof is orders of magnitude more sensitive at a higher mass resolution. An optional high mass quadrupole ion guide is available with a high mass cutoff of mz 100,000. The quadrupole ion guide has a high mass cutoff of mz 16,000. Quantof technology delivers superior uplccompatible mass resolution, matrixtolerant dynamic range, quantitative. The xevo g2xs qtof delivers not only conventional ms and msms methods of data acquisition, but also mse for comprehensive accurate mass precursor and fragment ion information from a single analysis, fastdda for rapid, intelligent automated accurate mass msms, and tofmrm for enhanced quantitative capability. Xevo g2xs tof performance, robustness, and flexibility made accessible to everyone. Intact quantification of trastuzumab is demonstrated using the xevo g2 xs qtof hrms and unifi scientific information system. View online or download waters xevo g2xs qtof overview and maintenance manual. Proteomics standards initiative list psidevmsdev archives. Xevo g2s qtof ms spray voltage 3 kv cone voltage 40 v source offset 80 v heated capillary temperature 250. The mass spectrometer analysis was performed on water xevo g2 qtofms waters, operating in the positive electrospray ionization mode. Muropeptide identity was confirmed by msms analysis, using a xevo g2dx qtof system waters corporation, usa. Wat introduces a new data acquisition mode, sonar, for its xevo g2xs quadrupole timeofflight qtof mass spectrometer.
The two probes are located at a 90 angle to each other. Nickelcatalyzed formal aminocarbonylation of secondary. These innovative technologies combine to provide the highest levels of tof performance. Xevo qtof mse xevo qtof msms qe aif qe msms ltqorbitrap msn figure 1. Ionbench with the waters xevo g2xs qtof mass spectrometer. Power off the external epc if it is a xevo g2 all other instrument epcs will power off with the electronics flip the electronics toggle switch down.
The xevo g2xs qtof is for research use only and is not intended for use in diagnostic applications. The difference in theoretical and observed monoisotropic masses in grams per mole was computed for each peak. Quantity of product ions and diagnostic product ions of nefazodone metabolites across platforms. This is the bottom of the two toggle switches at the back of the xevo wait a minute or two if you want you can reboot the pc during this time. Calibration fails on a qtof xevo or synapt due to poor. No communication with xevo g2xs wkb56990 how to clean the ion block on a xevo tqs, xevo g2s, xevo g2xs, synapt g2s, or synapt g2si style source. Waters advances proteomics and lipidomics research with novel. Xevo g2 qtof is equipped with a dual electrospray probe ion source that can spray a sample lc inlet and a calibration standard solution lock spray in a rapidly alternating manner. This is designed for use with both gas and liquid sample inlet device, specifically a dedicated uplc and api gc. Overview to scale the picture below is a simulation at scale of the waters xevo g2xs qtof lcms mass spectrometer system right side connected with an acquity uhplc left side, installed on a ionbench ms mass spec bench or table. Refurbished waters xevo g2 qtof pn 186005597 complete with esi source, pump and masslynx pc workstation. Another feature of the xevo g2xs qtof is the waters mass spectrometry technology, a patented.
Agilent technologies6200 series lc tof and the 6500 series lcq tof. How to do an electronic reboot on a xevo series qtof. Intact quantification of trastuzumab is demonstrated using the xevo g2xs qtof hrms and unifi scientific information system. Toddalia asiatica root bark as an effective hemostatic natural medicine or chinese materia medica was applied in china for long history, its complex drug action mechanisms and unclear substance basis have been constraining the development of this drug. Bch190ne58 detailed techniccal specifications noise enclosure for vacuum pumps noise reduction performance. Your quantitative and qualitative questions answered. Calibration or calibration check fails on a xevo or synapt qtof calibration runs but some peaks do not match and cannot be manually added only one peak may be matched peaks may be flagged with exclamation marks.
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